Effect of terminal 3'-hydroxymethyl modification of an RNA primer on nonenzymatic primer extension.
نویسندگان
چکیده
The significance of the precise position of the hydroxyl at the 3'-end of an RNA primer for nonenzymatic template-directed primer extension is not well understood. We show that an RNA primer terminating in 3'-hydroxymethyl-2',3'-dideoxy-guanosine has greatly diminished activity, suggesting that the spatial preorganization of the terminal sugar contributes significantly to the efficiency of primer extension.
منابع مشابه
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عنوان ژورنال:
- Chemical communications
دوره 52 80 شماره
صفحات -
تاریخ انتشار 2016